bxt buffer (IBA Lifesciences)

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IBA Lifesciences bxt buffer
Bxt Buffer, supplied by IBA Lifesciences, used in various techniques. Bioz Stars score: 96/100, based on 267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bxt+buffer/Buffer+BXT/pm42113987-255-21-23
Average 96 stars, based on 267 article reviews

bxt buffer - by Bioz Stars, 2026-09

96/100 stars

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Size-exclusion Chromatography:

Article Title: A Type VII-secreted toxin enables inter-mycobacterial competition
Article Snippet: .. The protein was eluted in BXT buffer (IBA), concentrated, and dialysed into 25 mM HEPES pH 7.5, 150 mM NaCl before size-exclusion chromatography with a HiLoad 26/600 200 pg column. .. E. coli T7 SHuffle (New England Biolabs) were transformed with 100 ng freshly prepped pET28a vector by heat shock and plated on Lysogeny Broth agar (VWR) plates supplemented with 50 μg/mL kanamycin.

Centrifugation:

Article Title: Refining the mechanism of heme acquisition from free hemoglobin by Staphylococcus aureus IsdH.
Article Snippet: Bacterial pathogens must acquire essential nutrients from the human host to survive and cause infection.. One of the most important is iron, which in our bloodstream is sequestered inside hemoglobin.. Staphylococcus aureus overcomes this barrier using surface proteins that capture hemoglobin and extract its heme cofactor.

Affinity Column:

Protease Inhibitor:

Article Title: Mitochondria-derived PEP licenses glycerolipid synthesis via SLC25A35
Article Snippet: The lysate was centrifuged at 21,000 rpm for 5 min and the supernatant was rotated with a Strep-TactinXT 4Flow high capacity resin (IBA, 2-5030-010) at 4°C for 3 hours. .. The resin was washed five times with W buffer containing 2% Triton X-100 and a protease inhibitor cocktail, followed by four rounds of rotation in BXT buffer (IBA, 2-1042-025) supplemented with 2% Triton X-100 and a protease inhibitor cocktail at 4°C for a total of 2 hours to purify the protein. .. For SLC25A35 mutant analysis, the codons TAT, AGA, and CGT were mutated to GCC to generate the Y124A, R175A, and R276A mutants, respectively, using an In-Fusion Cloning kit (Takara Bio).

Purification:

Article Title: The buried S2 apex of SARS-CoV-2 spike elicits an immunodominant germline-restricted public antibody response
Article Snippet: For CoV2-S spike proteins, purification was carried out using Strep-Tactin XT high-capacity columns (IBA Lifesciences). .. Proteins were eluted with BXT buffer (IBA Lifesciences) and further purified using a Superose 6 Increase column. ..

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